Search databaseBooksAll DatabasesAssemblyBiocollectionsBioProjectBioSampleBioSystemsBooksClinVarConserved DomainsdbGaPdbVarGeneGenomeGEO DataSetsGEO ProfilesGTRHomoloGeneIdentical Protein GroupsMedGenMeSHbeer-selection.com internet Sitebeer-selection.com CatalogNucleotideOMIMPMCPopSetProteinProtein ClustersProtein family ModelsPubChem BioAssayPubChem CompoundPubChem SubstancePubMedSNPSRAStructureTaxonomyToolKitToolKitAllToolKitBookgh

beer-selection.com Bookshelf. A company of the nationwide Library the Medicine, national Institutes the Health.

You are watching: What factor maintains the same distance between strands in a dna molecule?

Lodish H, Berk A, Zipursky SL, et al. Molecular cell Biology. Fourth edition. New York: W. H. Freeman; 2000.


By covenant with the publisher, this publication is accessible by the search feature, yet cannot be browsed.
\"*\"

DNA and RNA have an excellent chemical similarities. In their primarystructures both are direct polymers (multiple chemistry units) written of monomers (single chemical units), called nucleotides. Cellular RNAs range inlength from less than one hundreds to plenty of thousands the nucleotides. Cellular DNAmolecules deserve to be as long as number of hundred million nucleotides. These huge DNAunits in association with proteins deserve to be stained v dyes and visualized in thelight microscopic lense as chromosomes.


Polymerization that Nucleotides forms Nucleic Acids

DNA and RNA each consists of only four different nucleotides. All nucleotideshave a typical structure: a phosphate group connected by aphosphoester bond come a pentose (a five-carbon street molecule)that in turn is connected to an organic basic (Figure 4-1a). In RNA, the pentose isribose; in DNA, it is deoxyribose (Figure 4-1b). The only other distinction inthe nucleotides of DNA and RNA is that one of the 4 organic bases differsbetween the two polymers. The bases adenine, guanine, and cytosine are found inboth DNA and RNA; thymine is found only in DNA, and uracil is discovered only in RNA.The bases are often abbreviated A, G, C, T, and U, respectively. For conveniencethe single letters are also used when lengthy sequences that nucleotides room writtenout.


\"*\"

Figure 4-1

All Nucleotides have a usual structure. (a) Chemical structure of adenosine 5′-monophosphate (AMP),a nucleotide the is existing in RNA. All nucleotides are composed ofa phosphate moiety, containing increase to 3 phosphate groups, linkedto the (more...)


The base contents of main point acids are heterocyclic compounds v the ringscontaining nitrogen and also carbon. Adenine and guanine room purines, which contain a pair offused rings; cytosine, thymine, and uracil are pyrimidines, i m sorry contain a single ring (Figure 4-2). The acidic character ofnucleotides is because of the visibility of phosphate, i beg your pardon dissociates at the pHfound within cells, freeing hydrogen ions and leaving the phosphate negativelycharged (see number 2-22). Since thesecharges lure proteins, many nucleic mountain in cell are linked withproteins. In nucleotides, the 1′ carbon atom of the street (ribose ordeoxyribose) is attached to the nitrogen at position 9 the a purine(N9) or at position 1 the a pyrimidine (N1).


\"*\"

Figure 4-2

The chemical frameworks of the primary bases in nucleicacids. In main point acids and also nucleotides, nitrogen 9 the purines and also nitrogen1 the pyrimidines (red) room bonded to the 1′ carbon ofribose or deoxyribose.


Cells and also extracellular fluids in biology contain small concentrations the nucleosides, combine of a baseand a street without a phosphate. Nucleotides are nucleosides that have actually one, two,or three phosphate teams esterified in ~ the 5′ hydroxyl.Nucleoside monophosphates have actually a solitary esterifiedphosphate (see number 4-1a),diphosphates save on computer a prophosphate group


\"*\"

and triphosphates have a third phosphate. Table 4-1 perform the names of the nucleosides andnucleotides in main point acids and also the various develops of nucleoside phosphates. Aswe will check out later, the nucleoside triphosphates are used in the synthesis ofnucleic acids. However, these compounds likewise serve many other functions in thecell: ATP, for example, is the most widely used power carrier in the cell (seeFigure 2-25), and GTP theatre crucialroles in intracellular signaling and also acts as an energy reservoir, particularlyin protein synthesis.


\"*\"

When nucleotides polymerize to kind nucleic acids, the hydroxyl group attached tothe 3′ carbon the a sugar of one nucleotide develops an ester bond come thephosphate of an additional nucleotide, removed a molecule the water:


This condensation reaction is similar to the in i m sorry a peptide shortcut is formedbetween two amino acids (Chapter3). Therefore a solitary nucleic mountain strand is a phosphate-pentose polymer (apolyester) with purine and pyrimidine bases together side groups. The links betweenthe nucleotides are dubbed phosphodiesterbonds. Like a polypeptide, a nucleic mountain strand has actually an end-to-endchemical orientation: the 5′ finish hasa cost-free hydroxyl or phosphate group on the 5′ carbon that its terminalsugar; the 3′ end has a freehydroxyl team on the 3′ carbon of its terminal sugar (Figure 4-3). This directionality, add to thefact that synthesis proceeds 5′ to 3′, has given rise to theconvention that polynucleotide sequences room written and read in the5′ → 3′ direction (from left to right); forexample, the succession AUG is assumed to be (5′)AUG(3′).(Although, strictly speaking, the letter A, G, C, T, and also U stand for bases,they are additionally often used in diagrams to stand for the whole nucleotidescontaining these bases.) The 5′ → 3′directionality of a nucleic mountain strand is an extremely important residential property ofthe molecule.


Figure 4-3

Alternative methods of representing nucleic mountain chains, in thiscase a solitary strand the DNA containing just three bases: cytosine(C), adenine (A), and guanine (G). (a) Chemical framework of the trinucleotide CAG. Keep in mind the freehydroxyl group at the 3′ (more...)


The direct sequence of nucleotides attached by phosphodiester binding constitutes theprimary structure of nucleic acids. Together we comment on in the next section,polynucleotides can twist and also fold into three-dimensional conformationsstabilized through noncovalent bonds; in this respect, lock are comparable topolypeptides. Back the primary structures of DNA and also RNA are generallysimilar, their conformations are fairly different. Uneven RNA, which commonlyexists as a single polynucleotide chain, or strand, DNA consists of two intertwinedpolynucleotide strands. This structural difference is an important to the differentfunctions the the two species of nucleic acids.


Native DNA Is a dual Helix of safety Antiparallel Chains

The modern era of molecular biology started in 1953 when James D. Watson andFrancis H. C. Crick proposed appropriately the double-helical structure of DNA,based top top the evaluation of x-ray diffraction trends coupled with cautious modelbuilding. A closer look at the “thread that life,” as the DNAmolecule is periodically called, reflects why the discovery of its basic structuresuggests its function.

DNA is composed of two linked polynucleotide strands the windtogether through space to type a structure often describedas a double helix. The two sugar-phosphate backbones room on theoutside of the double helix, and the bases project right into the interior. Theadjoining bases in each strand ridge on peak of one an additional in parallel planes(Figure 4-4a). The orientation the thetwo strands is antiparallel; the is, their 5′ →3′ directions room opposite. The strands are organized in precise registerby a continual base-pairing between the two strands: A is paired v T throughtwo hydrogen bonds; G is paired v C through three hydrogen binding (Figure 4-4b). This base-paircomplementarity is a an effect of the size, shape, and also chemicalcomposition that the bases. The existence of countless such hydrogen bonds in aDNA molecule contributes significantly to the stability of the dual helix.Hydrophobic and van der Waals interactions in between the stacked surrounding basepairs likewise contribute come the security of the DNA structure.


Figure 4-4

Two depictions of contacts within the DNA doublehelix. (a) Space-filling version of B DNA, the many common type of DNA incells. The sugar and also phosphate residual water (gray) in every strand formthe backbone, i beg your pardon is traced through a red line, showing the helicaltwist (more...)


To keep the geometry the the double-helical structure presented in figure 4-4a, a larger purine (A or G) mustpair through a smaller sized pyrimidine (C or T). In organic DNA, A nearly alwayshydrogen bonds through T and G v C, creating A·T and also G·Cbase pairs often called Watson-Crick base pairs. Twopolynucleotide strands, or regions thereof, in which all the nucleotides formsuch base pairs are claimed to be complementary. However, in theory and in fabricated DNAs otherinteractions deserve to occur. For example, a guanine (a purine) might theoreticallyform hydrogen bonds through a thymine (a pyrimidine), bring about only a minordistortion in the helix. The an are available in the helix also would allowpairing in between the two pyrimidines cytosine and thymine. Although thenonstandard G·T and C·T basic pairs are normally not foundin DNA, G·U base pairs are quite typical in double-helical regionsthat form within otherwise single-stranded RNA.

Two polynucleotide strands can, in principle, form either a right-handed or aleft-handed helix (Figure 4-5). Becausethe geometry of the sugar-phosphate backbone is more compatible v the former,natural DNA is a right-handed helix. The x-ray diffraction sample of DNAindicates that the stacked bases are consistently spaced 0.34 nm apart along thehelix axis. The helix makes a finish turn every 3.4 nm; thus there space about10 pairs every turn. This is referred to as the B type of DNA,the normal form present in many DNA follow me in cells (Figure 4-6a). On the outside of B-form DNA, the spacesbetween the intertwined strands kind two helical grooves of various widthsdescribed as the major groove and also the minorgroove (see figure 4-4a). Consequently,part of each base is obtainable from outside the helix to both tiny and largemolecules that bind to the DNA through contacting chemical groups within the grooves.These 2 binding surface of the DNA molecule are offered by various classes ofDNA-binding proteins.


Figure 4-5

Two possible helical develops of DNA space mirror pictures of eachother. The geometry the the sugar-phosphate backbone the DNA causes naturalDNA to be right-handed. (Right-handed andleft-handed are defined by convention.)


Figure 4-6

Models of assorted DNA frameworks that are recognized to exist. The sugar-phosphate backbone of each chain is top top the external in allstructures (one red and also one blue) through the bases (silver) orientedinward. Side views are presented at the top, and views along the (more...)


In addition to the significant B form of DNA, three extr structures have beendescribed. In really low humidity, the crystallographic framework of B DNA changesto the A form; RNA-DNA and also RNA-RNA helices additionally exist in thisform. The A kind is an ext compact than the B form, having actually 11 bases per turn, andthe stack bases space tilted (Figure4-6b). Short DNA molecules created of alternate purine-pyrimidinenucleotides (especially Gs and also Cs) take on an alternate left-handedconfiguration rather of the typical right-handed helix. This structure is calledZ DNA because the bases seem to zigzag when viewed from theside (Figure 4-6c). That is entirelypossible that both A-form and also Z-form follow me of DNA exist in cells.

Finally, a triple-stranded DNA structure can additionally exist at least in the testtube, and also possibly throughout recombination and DNA repair. For example, whensynthetic polymers the poly(A) and also polydeoxy(U) space mixed, a three-strandedstructure is developed (Figure 4-6d).Further, long homopolymeric follow me of DNA composed of C and T residues in onestrand and also A and G residual water in the other can be target by brief matchinglengths of poly(C+T). The man-made oligonucleotide can insert together athird strand, binding in a sequence-specific path by so-calledHoogsteen base pairs. Certain cleavage the the DNA at thesite where the triple helix ends have the right to be completed by it is registered a chemicalcleaving agent (e.g., Fe2+-EDTA) come the shortoligodeoxynucleotide that makes up the third strand. Such reactions may beuseful in examining site-specific DNA damage in cells.

By far the most important adjustments in standard B-form DNA come around as aresult of protein binding to certain DNA sequences. Although the multitude ofhydrogen and also hydrophobic bonds in between the polynucleotide strands providestability to DNA, the double helix is somewhat flexible around its lengthy axis.Unlike the α helix in protein (see number 3-6), there are no hydrogen bonds between successive residuesin a DNA strand. This prop- erty allows DNA to bend when complexed v aDNA-binding protein. Crystallographic analyses of proteins bound to particularregions of DNA have actually conclusively prove departures from the standard B-DNAstructure in protein-DNA complexes. Two instances of DNA deformed by call withproteins are shown in figure 4-7. Thespecific DNA-protein contacts that happen in this tightly tied complexes havethe ability both to untwist the DNA and to bend the axis that the helix. AlthoughDNA in cells most likely exists in the B type most the the time, details regionsbound come protein plainly depart from the conventional conformation.


Figure 4-7

Bending that OF DNA result from protein binding. (a) A direct DNA (left) is presented binding a repressorprotein encoded by bacteriophage 434 (center); theresulting bend in the DNA (right) is conveniently seen bycomparison with the straight molecule. Binding (more...)


DNA deserve to Undergo Reversible Strand Separation

In DNA replication and also in the copying of RNA indigenous DNA, the strands of the helixmust different at the very least temporarily. Together we discuss later, during DNA synthesistwo brand-new strands space made (one copied from every of the initial strands),resulting in two twin helices similar with the original one. In the instance ofcopying the DNA layout to make RNA, the RNA is released and also the two DNAstrands reassociate v each other.

The unwinding and separation of DNA strands, described as denaturation, or“melting,” can be induced experimentally. For example, if asolution the DNA is heated, the thermal power increases molecule motion,eventually breaking the hydrogen bonds and also other pressures that stabilize thedouble helix, and the strands different (Figure4-8). This melt of DNA transforms its absorption of ultraviolet (UV)light (in the 260-nm range), which is routinely offered to measure up DNAconcentration because of the high absorbance of UV irradiate by nucleic acid bases.Native double-stranded DNA absorbs around one-half as much light in ~ 260 nm asdoes the equivalent amount the single-stranded DNA (Figure 4-9a). Thus, as DNA denatures, its absorption of UVlight increases. Near the denaturation temperature, a small increase intemperature reasons an abrupt, near simultaneous, loss of the multiple, weak,cooperative interaction holding the 2 strands together, so the denaturationrapidly occurs throughout the entire length of the DNA.


Figure 4-9

Light absorption and also temperature in DNA denaturation. (a) melting of doubled-stranded DNA deserve to be monitored by theabsorption of ultraviolet irradiate at 260 nm. As regions ofdouble-stranded DNA unpair, the absorption of light by those regionsincreases almost (more...)


The melting temperature, Tm, at which the strands ofDNA will separate counts on several factors. Molecules that contain a greaterproportion of G·C bag require higher temperatures to denaturebecause the 3 hydrogen bonds in G·C pairs do them more stablethan A·T pairs with two hydrogen bonds (see number 4-4b). Indeed, the percent of G·Cbase pairs in a DNA sample can be estimated from itsTm (Figure4-9b). In enhancement to heat, remedies of low ion concentrationdestabilize the double helix, resulting in it to melt at lower temperatures. DNA isalso denatured by exposure to other agents that destabilize hydrogen bonds, suchas alkaline solutions and concentrated remedies of formamide or urea:


The single-stranded DNA molecules that an outcome from denaturation form random coilswithout a regular structure. Lowering the temperature or enhancing the ionconcentration reasons the two complementary strands come reassociate right into a perfectdouble helix (see number 4-8). The extentof such renaturation is dependence on time, the DNAconcentration, and the ionic contents of the solution. 2 DNA strands notrelated in sequence will continue to be as random coils and will no renature and, mostimportant, will certainly not considerably inhibit safety DNA companion strands fromfinding every other. Denaturation and also renaturation that DNA room the basis ofnucleic mountain hybridization, apowerful method used to research the relatedness of two DNA samples and also todetect and also isolate details DNA molecules in a mixture comprise numerousdifferent DNA order (Chapter7).


Many DNA Molecules space Circular

All prokaryotes genomic DNAs and many viral DNAs space circular molecules. CircularDNA molecules also occur in mitochondria, i m sorry are existing in virtually alleukaryotic cells, and also in chloroplasts, which are present in plants and someunicellular eukaryotes.

Each that the two strands in a one DNA molecule develops a close up door structurewithout complimentary ends. Simply as is the situation for linear DNA, elevated temperature oralkaline pH destroy the hydrogen bonds and also other interactions the stabilizedouble-helical circular DNA molecules. Unlike direct DNA, however, the twostrands of circular DNA can not unwind and also separate; attempts to melt together DNAresult in one interlocked, tangled fixed of single-stranded DNA (Figure 4-10a).


Figure 4-10

Denaturation of circular DNA. (a) If both strands are closed circles, denaturation disrupts thedouble helix, however the two solitary strands become tangled around eachother and cannot separate. (b) If one or both strands room nicked,however, the two strands (more...)


Only if a native circular DNA is nicked (i.e., among thestrands is cut), will certainly the 2 strands unwind and separate when the molecule isdenatured. In this case, one of the separated strands is circular, and also the otheris straight (Figure 4-10b). Nicking ofcircular DNA occurs naturally during DNA replication and can it is in inducedexperimentally with a low concentration of deoxyribonuclease (a DNA-degradingenzyme), therefore that only a single phosphodiester bond in the molecule is cleaved.The research of circular DNA molecules lacking complimentary ends first uncovered thecomplicated geometric shape alters that the double-stranded DNA molecule mustundergo when the strands room not cost-free to separate.


Local Unwinding the DNA induces Supercoiling

So much we have defined DNA as a long constant helical structure that can havelocal perturbations, especially due to protein binding. In addition, as soon as thetwo ends of a DNA molecule room fixed, the molecule exhibits a superstructureunder specific conditions. This occurs once the base pairing is interrupted and also alocal an ar unwinds. The tension induced by unwinding is relieved through twisting ofthe double helix top top itself, forming supercoils (Figure 4-11). Unwinding and subsequentsupercoiling occurs during replication, transcription, and also binding the manyproteins to circular DNAs or to lengthy DNA loops whose end are solved withineukaryotic chromosomes. Supercoiling is recognized and regulated through enzymescalled topoisomerases. As discussedin later on chapters, these enzymes have an essential role in both DNA replicationand the warrior of DNA into RNA.


Figure 4-11

Supercoiling in electron micrographs the DNA isolated native theSV40 virus. Once isolated SV40 DNA is separated native its associated protein, theDNA duplex is underwound and also assumes the supercoiled configuration(form I). If one strand is nicked, the strands (more...)


RNA molecules Exhibit varied Conformations and also Functions

As provided earlier, the main structure the RNA is generally comparable to the ofDNA; however, the sugar component (ribose) the RNA has secondary hydroxylgroup at the 2′ place (see Figure4-1b), and thymine in DNA is replaced by uracil in RNA (see figure 4-2). The hydroxyl group onC2 that ribose provides RNA more chemically labile than DNA andprovides a chemically reactive team that takes component in RNA-mediated enzymaticevents. Together a result of this lability, RNA is cleaved right into mononucleotides byalkaline solution, conversely, DNA is not. Favor DNA, RNA is a long polynucleotidethat deserve to be double-stranded or single-stranded, straight or circular. It deserve to alsoparticipate in a hybrid helix created of one RNA strand and one DNA strand;this hybrid has actually a slightly different conformation than the typical B kind ofDNA.

Unlike DNA, i m sorry exists generally in a single, an extremely long three-dimensionalstructure, the double helix, the various species of RNA exhibit differentconformations. Differences in the sizes and conformations that the assorted typesof RNA allow them to lug out details functions in a cell. The simplestsecondary frameworks in single-stranded RNAs are created by pairing ofcomplementary bases. “Hairpins” are created by pairing ofbases within ≈5 – 10 nucleotides of eachother, and “stem-loops” through pairing the bases the areseparated by ≈50 to several hundred nucleotides (Figure 4-12a). These straightforward folds cancooperate to type more facility tertiary structures, among which is termed a“pseudoknot” (Figure4-12b).


Figure 4-12

RNA secondary and tertiary structures. (a) Stem-loops, hairpins, and other an additional structures can kind bybase pairing between distant complementary segment of one RNAmolecule. In stem-loops, the single-stranded loop (dark red) betweenthe base-paired (more...)


As disputed in information later, tRNA molecules adopt a well-definedthree-dimensional architecture in systems that is vital in protein synthesis.Larger rRNA molecules also have in your ar well identified three-dimensionalstructures, with much more flexible web links in between. Secondary and tertiarystructures likewise have been recognized in mRNA, an especially near the end ofmolecules. This recently discovered structures are under energetic study. Clearly,then, RNA molecules are prefer proteins in that they have structured domainsconnected by less structured, versatile stretches.

The folded domains of RNA molecule not just are structurally analogous to theα helices and also β strands uncovered in proteins, yet in some casesalso have actually catalytic capacities. Together catalytic RNAs, dubbed ribozymes, can reduced RNA chains. SomeRNA domains additionally can catalyze RNA splicing, a remarkableprocess in i m sorry an internal RNA sequence, an intron, is cut and removed and the two resulting chains,the exons, room sealed together.This process occurs during formation of the majority of useful mRNAmolecules in eukaryotic bio cells, and also occurs in bacteria and also archaea.Remarkably, part RNAs carry out self-splicing, with thecatalytic task residing in the intron sequence. The instrument of splicingand self-splicing are discussed in information in chapter 11. As detailed later in this chapter, rRNA isthought come play a catalytic duty in the formation of peptide bond duringprotein synthesis.

In this chapter, we emphasis on the attributes of mRNA, tRNA, and rRNA in geneexpression — the process of getting theinformation in DNA converted into proteins. In later on chapters we will encounterother RNAs, often associated with proteins, that participate in other cellfunctions.

See more: Is Zip Code Categorical Or Quantitative, Is Zip Code Quantitative Or Categorical


By agreement with the publisher, this book is accessible by the find feature, however cannot be browsed.