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LAB: OSMOSIS IN A RED ONION CELL-DO NOT PRINT THIS PAGE Cytoplasm = very thin white area between vacuole and cell wellLarge Central Vacuole = pink area inside cellCytoplasm = clear area around vacuoleCell Wall = grayish edges around cellRed onion cell in distilled water 400XRed onion cell in salt water 400XDO NOT PRINT THIS PAGE—USE IT AS A REFERENCE TO DRAW THE PICTURES AND ANSWER THE QUESTIONSFor your lab, pretend this is what you see under the microscope. Step 1. After you label the magnification as 400x,  DRAW the image of the red onion cell in distilled water (use the circles on your data page)—don’t forget your title! It needs to look exactly like image—fill your circle!!  LABEL the Large Central Vacuole and Cell wall! Step 2: After you label the magnification as 400x,  DRAW the image of the red onion cell in salt water (use the circles on your data page)—don’t forget your title! It needs to look exactly like image—fill your circle!!  LABEL the Large Central Vacuole and Cell wall!  What happened? ….. Step 3: Complete the lab by answering the questions on the student data page. You will need your textbook and your 7-3 notes. DRAWINGS NEED TO BE NEAT, DETAILED, AND IN COLOR!LAB: OSMOSIS IN AN ONION CELL Student Data Page RESULTS *Use pencil or colored pencil. NO DETAIL; NO CREDIT! Figure 1: _________________________ Magnification: ___________________Figure 2: _________________________ Magnification: ___________________LAB QUESTIONS 1. What is the difference between osmosis and diffusion? 2. Why aren’t your cells constantly bursting (lysing) if there is a build up of pressure inside the cell (osmotic pressure) due to the solute concentration?3. DRAW and EXPLAIN what happens in a hypertonic solution? (look at your poster)4. In this lab, are we creating a hypotonic or hypertonic environment when we add the salt water to the onion cells (explain your answer in detail)?5. What is the function of the central vacuole in plants?6. Why doesn’t the cell membrane dissolve when it is in the presence of water (think about the function of the cell membrane and what it is made of………..look at your notes)7. Describe what happened to the onion cells when you soaked them in salt water. (Describe the movement of water and what happened to the vacuole.)8.

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Which caused greater osmotic pressure inside the cell, the distilled water solution or the saltwater solution? (See page 187 for extra information on osmotic pressure.)9.In the plant cell, what prevents the cell from bursting (lysing)?10. If we watered whole onion plants growing in the field with salt water, how would the plants respond? (Explain what would happen to the onion cells and the overall effect on the plant—look in your notes for help.)